investigation of flk-1 gene expression in differentiated mesenchymal stem cells, exposed to chemical, mechanical and chemical-mechanical factors, in order to study the differentiation and its stability

نویسندگان

e hasanzadeh department of life sciences engineering, faculty of new sciences & technologies, university of tehran, tehran, iran.

gh amoabediny research center for new technologies in life science engineering, university of tehran, tehran, iran.

n haghighipour national cell bank of iran, pasteur institute of iran, tehran, iran.

n amirzadeh blood research center, high institute for education and research in transfusion medicine, tehran, iran.

چکیده

background: mesenchymal stem cells (mscs) are multipotent cells, capable of differentiating into different cell lines.they can sense their surrounding biochemical and biophysical factors, which play major roles in their differentiation toward different phenotypes. therefore, the exposure of these cells to endothelial growth factor (vegf) as well as hemodynamic biomechanical forces, which act on endothelial cells in vivo, may direct mscs toward vascular covering endothelial cells. the aim of this study, is to investigate the effects of chemical, mechanical and chemical-mechanical factors on the differentiation of mesenchymal stem cells and to examine the stability of this in vitro differentiation. materials and method: human subcutaneous adipose-derived mesenchymal stem cells were cultured in dmem containing 20% fbs. they were characterized in passage 2, using flow cytometery technique and then were used in experiments. mesenchymal stem cells were cultured in tubular silicone scaffolds, and then exposed to endothelial growth factor (vege), shear stress or combination of these two signals. in order to examine the differentiation, the expression level of flk-1, as an endothelial specific gene, was studied. for studying the stability of this differentiation, flk-1 mrna levels were quantified using real-time pcr, upon completion of experiments, as well as on the 5thand 10th days after the tests. results: according to the results of real-time pcr, immediately upon the completion of tests, mrna level of flk-1 was higher in the mechanical group (5.44), compared to the chemical (1.18) and chemical-mechanical (2.65) ones. on the 5th and 10th days after the experiments, flk-1 expression levels were higher in chemical-mechanical group (6.34 and 7.56 respectively), compared to the chemical (2.18 and 0.99, respectively), and mechanical (2.96 and 3.92, respectively) ones. conclusion: the findings of this study demonstrate that not only flk-1 gene expression is stable over time, but also cells exposed to the chemical and mechanical cues, simultaneously, show the highest expression of this gene. the increase in flk-1 expression over time implies the enhanced compatibility of differentiated cells with each other and their surrounding environment, which may be probably due to the higher sensitivity of flk-1 gene expression to the combination of chemical and mechanical signals.   keywords: differentiation, shear stress, stem cell, stability, vegf.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Investigation of FLK-1 Gene Expression in Differentiated Mesenchymal Stem Cells, Exposed to Chemical, Mechanical and Chemical-mechanical Factors, in order to Study the Differentiation and its Stability

Background: Mesenchymal stem cells (MSCs) are multipotent cells, capable of differentiating into different cell lines.They can sense their surrounding biochemical and biophysical factors, which play major roles in their differentiation toward different phenotypes. Therefore, the exposure of these cells to endothelial growth factor (VEGF) as well as hemodynamic biomechanical forces, which act on...

متن کامل

a frame semantic approach to the study of translating cultural scripts in salingers franny and zooey

the frame semantic theory is a nascent approach in the area of translation studies which goes beyond the linguistic barriers and helps us to incorporate cognitive and cultural factors to the study of translation. based on rojos analytical model (2002b), which centered in the frames or knowledge structures activated in the text, the present research explores the various translation problems that...

15 صفحه اول

contribution of setting to the study of characters and the main idea in e. brontes: wuthering heights and t. hardys: return of the native.

this thesis aims to adduce an unmitigated and comprehensive explication concerning the relationship of three significant elements of fiction: setting, chracter and theme. my research is basically placed on two outstanding novels of the 19th century: emily brontes wuthering heights and thomas hardys return of the native. my endeavour lies in studying the correlation among the three above-mention...

15 صفحه اول

identifying the strategies persian efl learners use in reading an expository text in english and examining its relation to reading-proficiency and motivation: a think-aloud study

هدف اصلی از این مطالعه بررسی نوع و میزان استراتژی هایی بود که دانشجویان فارسی زبان رشته ی زبان انگلیسی در حین خواندن یک متن انگلیسی به کار گرفتند. این مطالعه همچنین به بررسی تفاوت های استراتژی های مورد استفاده بین دارندگان سطح بالا و پایین درک مطلب پرداخت. نوع همبستگی بین استراتژی به کار گرفته و درک مطلب از یک سو و استراتژی به کار گرفته و انگیزه از سوی دیگر نیز در این تحقیق مورد آزمایش قرار گرف...

15 صفحه اول

the study of aaag repeat polymorphism in promoter of errg gene and its association with the risk of breast cancer in isfahan region

چکیده: سرطان پستان دومین عامل مرگ مرتبط با سرطان در خانم ها است. از آنجا که سرطان پستان یک تومور وابسته به هورمون است، می تواند توسط وضعیت هورمون های استروئیدی شامل استروژن و پروژسترون تنظیم شود. استروژن نقش مهمی در توسعه و پیشرفت سرطان پستان ایفا می کند و تاثیر خود را روی بیان ژن های هدف از طریق گیرنده های استروژن اعمال می کند. اما گروه دیگری از گیرنده های هسته ای به نام گیرنده های مرتبط به ا...

15 صفحه اول

the role of russia in transmission of energy from central asia and caucuses to european union

پس ازفروپاشی شوروی،رشد منابع نفت و گاز، آسیای میانه و قفقاز را در یک بازی ژئوپلتیکی انرژی قرار داده است. با در نظر گرفتن این منابع هیدروکربنی، این منطقه به یک میدانجنگ و رقابت تجاری برای بازی های ژئوپلتیکی قدرت های بزرگ جهانی تبدیل شده است. روسیه منطقه را به عنوان حیات خلوت خود تلقی نموده و علاقمند به حفظ حضورش می باشد تا همانند گذشته گاز طبیعی را به وسیله خط لوله مرکزی دریافت و به عنوان یک واس...

15 صفحه اول

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
international journal of pediatrics

جلد ۲، شماره ۲.۳، صفحات ۹۰-۹۰

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023